ROOM (Regulatory On/Off Minimization) 
Regulatory on/off minimization (ROOM) is a constraint-based algorithm for
predicting the metabolic steady state after gene knockouts. It aims to
minimize the number of significant flux changes (hence onoff) with respect
to the wild type.
Regulatory On/Off Minimization Of Metabolic Flux Changes After Genetic Perturbations.
T. Shlomi, O. Berkman and E. Ruppin.